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Site specificity of glycation and carboxymethylation of bovine serum albumin by fructose

Hinton, D.J.S. and Ames, J.M. (2006) Site specificity of glycation and carboxymethylation of bovine serum albumin by fructose. Amino Acids, 30 (4). pp. 425-433. ISSN 0939-4451

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To link to this item DOI: 10.1007/s00726-006-0269-2


We report an investigation of the site specificity, extent and nature of modification of bovine serum albumin (BSA) incubated with fructose or glucose at physiological temperature and pH. Sites of early glycation (Heyns rearrangement products (HRP) from fructose; fructoselysine (FL) from glucose) as well as advanced glycation (N-epsilon-(carboxymethyl)lysine; CML) wereanalyzed by liquid chromatography-mass spectrometry. The major site of modification by fructose, like glucose, is Lysine-524 and this results in, respectively, 31 and 76% loss of the corresponding unmodified tryptic peptide, Gln525-Lys533. In addition, total lysine, HRP, FL, CML and N-epsilon-(carboxyethyl)lysine in the incubations, was quantified. Almost all of the loss of lysine in the fructose-modified BSA was attributed to the formation of CML, with the yield of CML being up to 17-fold higher than glucose-modified BSA. A mechanism for the formation of CML from the HRP is proposed.

Item Type:Article
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13316
Uncontrolled Keywords:fructose, advanced glycation endproduct, site specificity, fructoselysine, Heyns rearrangement products, N-delta-(carboxymethyl)lysine, N-EPSILON-CARBOXYMETHYLLYSINE, AGE-DEPENDENT ACCUMULATION, END-PRODUCTS, MASS-SPECTROMETRY, MAILLARD REACTION, PROTEOMIC ANALYSIS, DIABETES-MELLITUS, LENS PROTEINS, GLUCOSE, IDENTIFICATION

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