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Cotham, W.E., Hinton, D.J.S., Metz, T.O., Brock, J.W.C., Thorpe, S.R., Baynes, J.W. and Ames, J.M. (2003) Mass spectrometric analysis of glucose-modified ribonuclease. Biochemical Society Transactions, 31 (6). pp. 1426-1427. ISSN 0300-5127
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Official URL: http://www.biochemsoctrans.org
Abstract/Summary
RNase A (1 mM) was incubated with glucose (0.4 M) at 37°C for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys1, Lys7, Lys37 and Lys41. Three of these sites (Lys7, Lys37 and Lys41) were also the major sites of Ne-(carboxymethyl)lysine formation.
| Item Type: | Article |
|---|---|
| Refereed: | Yes |
| Divisions: | Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences |
| ID Code: | 13482 |
| Uncontrolled Keywords: | advanced glycation end product (AGE), Ne-(carboxymethyl)lysine, fructoselysine, glucose, LC-MS, RNase. |
| Additional Information: | Biochemical Society Main Meeting 679th Meeting University of Essex, Colchester, UK 2–4 July 2003 |
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