Development of a miniaturised microarray-based assay for the rapid identification of antimicrobial resistance genes in Gram-negative bacteria

Batchelor, M., Hopkins, K. L., Liebana, E., Slickers, P., Ehricht, R., Mafura, M., Aarestrup, F., Mevius, D., Clifton-Hadley, F. A., Woodward, M. J., Davies, R. H., Threlfall, E. J. and Anjum, M. F. (2008) Development of a miniaturised microarray-based assay for the rapid identification of antimicrobial resistance genes in Gram-negative bacteria. International Journal of Antimicrobial Agents, 31 (5). pp. 440-451. ISSN 0924-8579 doi: 10.1016/j.ijantimicag.2007.11.017

Abstract/Summary

We describe the development of a miniaturised microarray for the detection of antimicrobial resistance genes in Gram-negative bacteria. Included on the array are genes encoding resistance to aminoglycosides, trimethoprim, sulphonamides, tetracyclines and beta-lactams, including extended-spectrum beta-lactamases. Validation of the array with control strains demonstrated a 99% correlation between polymerase chain reaction and array results. There was also good correlation between phenotypic and genotypic results for a large panel of Escherichia coli and Salmonella isolates. Some differences were also seen in the number and type of resistance genes harboured by E. coli and Salmonella strains. The array provides an effective, fast and simple method for detection of resistance genes in clinical isolates suitable for use in diagnostic laboratories, which in future will help to understand the epidemiology of isolates and to detect gene linkage in bacterial populations. (C) 2008 Published by Elsevier B.V. and the International Society of Chemotherapy.