Type III secretion homologs are present in Brucella melitensis, B-ovis, and B-suis biovars 1, 2, and 3Abdallah, A. I., Commander, N. J., Woodward, M. J., Spencer, S., Hart, C. A. and Winstanley, C. (2003) Type III secretion homologs are present in Brucella melitensis, B-ovis, and B-suis biovars 1, 2, and 3. Current Microbiology, 46 (4). pp. 241-245. ISSN 0343-8651 Full text not archived in this repository. It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing. To link to this item DOI: 10.1007/s00284-002-3789-3 Abstract/SummaryProtein sequences from characterized type III secretion (TTS) systems were used as probes in silico to identify several TTS gene homologs in the genome sequence of Brucella suis biovar 1 strain 1330. Four of the genes, named flhB, fliP, fliR, and fliF on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in PCR and hybridization assays to determine their distribution among other Brucella nomen species and biovars. The results indicated that flhB, fliP, fliR and fliF are present in Brucella melitensis, Brucella ovis, and Brucella suis biovars 1, 2 and 3. Similar homologos have been reported previously in Brucella abortus. Using RT-PCR assays, we were unable to detect any expression of these genes. It is not yet known whether the genes are the cryptic remnants of a flagellar system or are actively involved in a process contributing to pathogenicity or previously undetected motility, but they are distributed widely in Brucella and merit further study to determine their role.
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