Simple and effective technical procedures for the packing and application of nanospray PicoFrit HPLC columns for proteomic analyses

Howells, L. C., Whelan, A. O., Woodward, M. J. and Coldham, N. G. (2005) Simple and effective technical procedures for the packing and application of nanospray PicoFrit HPLC columns for proteomic analyses. Proteomics, 5 (15). pp. 3864-3867. ISSN 1615-9853 doi: 10.1002/pmic.200401259

Abstract/Summary

A simple procedure was developed for packing PicoFrit HPLC columns with chromatographic stationary phase using a reservoir fabricated from standard laboratory HPLC fittings. Packed columns were mounted onto a stainless steel ultra-low volume precolumn filter assembly containing a 0.5-mu m pore size steel frit. This format provided a conduit for the application of the nanospray voltage and protected the column from obstruction by sample material. The system was characterised and operational performance assessed by analysis of a range of peptide standards (n = 9).