Accessibility navigation

Deleterious effects of calcium indicators within cells: an inconvenient truth

Bootman, M. D., Allman, S., Rietdorf, K. and Bultynck, G. (2018) Deleterious effects of calcium indicators within cells: an inconvenient truth. Cell Calcium, 73. pp. 82-87. ISSN 0143-4160

Full text not archived in this repository.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1016/j.ceca.2018.04.005


The study of cellular Ca2+ signalling is indebted to Roger Tsien for the invention of fluorescent indicators that can be readily loaded into living cells and provide the means to measure cellular Ca2+ changes over long periods of time with sub-second resolution and microscopic precision. However, a recent study [1] reminds us that as useful as these tools are they need to be employed with caution as there can be off-target effects. This article summarises these recent findings within the wider context of confounding issues that can be encountered when using chemical and genetically-encoded Ca2+ indicators, and briefly discusses some approaches that may mitigate against misleading outcomes.

Item Type:Article
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Medicinal Chemistry Research Group
ID Code:78122

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation