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Precise quantification of platelet proteins and their phosphorylation states

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Mazet, F. and Fry, M. J. (2018) Precise quantification of platelet proteins and their phosphorylation states. In: Gibbins, J. M. ORCID: https://orcid.org/0000-0002-0372-5352 and Mahaut-Smith, M. (eds.) Platelets and Megakaryocytes: Volume 4, Advanced Protocols and Perspectives. Humana Press, New York, pp. 113-125. ISBN 9781493985845

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To link to this item DOI: 10.1007/978-1-4939-8585-2_7

Abstract/Summary

Systems biology and modeling approaches require quantitative data-rich temporal experiments to better understand the dynamics and regulation of the components of the signaling pathways that governs cell biology and physiology. Here we present a modified Western blotting method to rapidly analyze and accurately quantify protein copy number, and their respective phosphorylation states at specific sites over detailed time courses.

Item Type:Book or Report Section
Refereed:No
Divisions:Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
ID Code:79193
Uncontrolled Keywords:High-density, Immunoblotting, Protein quantification, Western
Publisher:Humana Press
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