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Speciation and milk adulteration analysis by rapid ambient liquid MALDI mass spectrometry profiling using machine learning

Piras, C., Hale, O. J., Reynolds, C. K. ORCID: https://orcid.org/0000-0002-4152-1190, Jones, A. K., Taylor, N., Morris, M. and Cramer, R. ORCID: https://orcid.org/0000-0002-8037-2511 (2021) Speciation and milk adulteration analysis by rapid ambient liquid MALDI mass spectrometry profiling using machine learning. Scientific Reports, 11 (3305). ISSN 2045-2322 (In Press)

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To link to this item DOI: 10.1038/s41598-021-82846-5

Abstract/Summary

Growing interest in food quality and traceability by regulators as well as consumers demands advances in more rapid, versatile and cost-effective analytical methods. Milk, as most food matrices, is a heterogeneous mixture composed of metabolites, lipids and proteins. One of the major challenges is to have simultaneous, quantitative detection (profiling) of this panel of biomolecules to gather valuable information for assessing food quality, traceability and safety. Here, for milk analysis, atmospheric pressure (AP) matrix-assisted laser desorption/ionization (MALDI) employing homogenous liquid sample droplets was used on a Q-TOF mass analyzer. This method has the capability to produce multiply charged proteinaceous ions as well as highly informative profiles of singly charged lipids/metabolites. In two examples, this method is coupled with user-friendly machine-learning software. First, rapid speciation of milk (cow, goat, sheep and camel) is demonstrated with 100% classification accuracy. Second, the detection of cow milk as adulterant in goat milk is shown at concentrations as low as 5% with 92.5% sensitivity and 94.5% specificity.

Item Type:Article
Refereed:Yes
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
ID Code:95632
Publisher:Nature Publishing Group

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