Accessibility navigation

Raw cow milk bacterial consortium as bioindicator of circulating anti-microbial resistance (AMR)

Piras, C., Greco, V., Gugliandolo, E., Soggiu, A., Tilocca, B., Bonizzi, L., Zecconi, A., Cramer, R. ORCID:, Britti, D., Urbani, A. and Roncada, P. (2020) Raw cow milk bacterial consortium as bioindicator of circulating anti-microbial resistance (AMR). Animal Feed Science and Technology, 10 (12). 2378. ISSN 0377-8401

Text (Open Access) - Published Version
· Available under License Creative Commons Attribution.
· Please see our End User Agreement before downloading.


It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.3390/ani10122378


The environment, including animals and animal products, is colonized by bacterial species that are typical and specific of every different ecological niche. Natural and human-related ecological pressure promotes the selection and expression of genes related to antimicrobial resistance (AMR). These genes might be present in a bacterial consortium but might not necessarily be expressed. Their expression could be induced by the presence of antimicrobial compounds that could originate from a given ecological niche or from human activity. In this work, we applied (meta)proteomics analysis of bacterial compartment of raw milk in order to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition. Results from milk analysis revealed the presence of 29 proteins/proteoforms linked to AMR. The detection of mainly β-lactamases suggests the possibility of using the milk microbiome as a bioindicator for the investigation of AMR. Moreover, it was possible to achieve a culture-free qualitative and functional analysis of raw milk bacterial consortia.

Item Type:Article
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
ID Code:95667


Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation