Accessibility navigation


Selective fermentation of gentiobiose-derived oligosaccharides by human gut bacteria and influence of molecular weight

Sanz, M.L., Cote, G.L., Gibson, G.R. and Rastall, R.A. (2006) Selective fermentation of gentiobiose-derived oligosaccharides by human gut bacteria and influence of molecular weight. Fems Microbiology Ecology, 56 (3). pp. 383-388. ISSN 0168-6496

Full text not archived in this repository.

To link to this article DOI: 10.1111/j.1574-6941.2006.00075.x

Abstract/Summary

Gentiooligosaccharides and alternansucrase gentiobiose acceptor products were fractionated by their degree of polymerization (DP) on a Bio-Gel P2 column. Fractions were characterized by matrix-assisted laser desorption ionization time-of-flight mass spectroscopy, and incubated with human faecal bacteria under anaerobic conditions at 37 degrees C. The growth of predominant gut bacteria on the oligosaccharides was evaluated by fluorescence in situ hybridization and a prebiotic index (PI) was calculated. Lower DP gentiooligosaccharides (DP2-3) showed the highest selectivity (PI of 4.89 and 3.40, respectively), whereas DP4-5 alternansucrase gentiobiose acceptor products generated the greatest values (PI of 5.87). The production of short-chain fatty acids was also determined during the time course of the reactions. The mixture of DP6-10 alternansucrase gentiobiose acceptor products generated the highest levels of butyric acid but the lowest levels of lactic acid. Generally, for similar molecular weights, alternansucrase gentiobiose acceptor products gave higher PI values than gentiooligosaccharides.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13010
Uncontrolled Keywords:prebiotic, gentiobiose, oligosaccharide fermentation, alternansucrase , MESENTEROIDES NRRL B-1355, HUMAN COLONIC MICROBIOTA, IN-SITU HYBRIDIZATION, OLIGONUCLEOTIDE PROBES, DIETARY MODULATION, HUMAN FECES, ALTERNANSUCRASE, VITRO, PREBIOTICS

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation