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Protein-lipid interactions at the air/water interface

Lad, M.D., Birembaut, F., Frazier, R.A. and Green, R.J. (2005) Protein-lipid interactions at the air/water interface. Physical Chemistry Chemical Physics, 7 (19). pp. 3478-3485. ISSN 1463-9076

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To link to this article DOI: 10.1039/b506558p

Abstract/Summary

Surface pressure measurements and external reflection FTIR spectroscopy have been used to probe protein-lipid interactions at the air/water interface. Spread monomolecular layers of stearic acid and phosphocholine were prepared and held at different compressed phase states prior to the introduction of protein to the buffered subphase. Contrasting interfacial behaviour of the proteins, albumin and lysozyme, was observed and revealed the role of both electrostatic and hydrophobic interactions in protein adsorption. The rate of adsorption of lysozyme to the air/water interface increased dramatically in the presence of stearic acid, due to strong electrostatic interactions between the negatively charged stearic acid head group and lysozyme, whose net charge at pH 7 is positive. Introduction of albumin to the subphase resulted in solubilisation of the stearic acid via the formation of an albumin-stearic acid complex and subsequent adsorption of albumin. This observation held for both human and bovine serum albumin. Protein adsorption to a PC layer held at low surface pressure revealed adsorption rates similar to adsorption to the bare air/water interface and suggested very little interaction between the protein and the lipid. For PC layers in their compressed phase state some adsorption of protein occurred after long adsorption times. Structural changes of both lysozyme and albumin were observed during adsorption, but these were dramatically reduced in the presence of a lipid layer compared to that of adsorption to the pure air/water interface.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy
ID Code:13232
Uncontrolled Keywords:AIR-WATER-INTERFACE, PHOSPHOLIPID MONOLAYERS, REFLECTION-ABSORPTION, INFRARED REFLECTION, CYTOCHROME-C, SPECTROSCOPY, ADSORPTION, LYSOZYME, PHOSPHATIDYLCHOLINE, ASSOCIATION
Additional Information:228th National Meeting of the American-Chemical-Society Philadelphia, Pennsylvania, USA 22-26 AUG 2004

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