Accessibility navigation


In vitro fermentation of sugar beet arabinan and arabino-oligosaccharides by the human gut microflora

Al-Tamimi, M.A.H.M., Palframan, R.J., Cooper, J.M., Gibson, G.R. and Rastall, R.A. (2006) In vitro fermentation of sugar beet arabinan and arabino-oligosaccharides by the human gut microflora. Journal of Applied Microbiology, 100 (2). pp. 407-414. ISSN 1364-5072

Full text not archived in this repository.

To link to this article DOI: 10.1111/j.1365-2672.2005.02780.x

Abstract/Summary

Aims: To determine the fermentation profiles by human gut bacteria of arabino-oligosaccharides of varying degree of polymerization. Materials and Methods: Sugar beet arabinan was hydrolyzed with a commercial pectinase and eight fractions, of varying molecular weight, were isolated by gel-filtration chromatography. Hydrolysis fractions, arabinose, arabinan and fructo-oligosaccharides were fermented anaerobically by gut bacteria. Total bacteria, bifidobacteria, bacteroides, lactobacilli and the Clostridium perfringens/histolyticum sub. grp. were enumerated using fluorescent in situ hybridization. Results: Bifidobacteria were stimulated to different extents depending on molecular weight, i.e. maximum increase in bifidobacteria after 48 h was seen on the lower molecular weight fractions. Lactobacilli fluctuated depending on the initial inoculum levels. Bacteroides numbers varied according to fraction; arabinan, arabinose and higher oligosaccharides (degree of polymerization, dp > 8) resulted in significant increases at 24 h. Only carbohydrate mixtures with dp of 1-2 resulted in significant increases at 48 h (log 8.77 +/- 0.23). Clostridia decreased on all substrates. Conclusions: Arabino-oligosaccharides can be considered as potential prebiotics. Significance and Impact of the Study: Arabinan is widely available as it is a component of sugar beet pulp, a co-product from the sugar beet industry. Generation of prebiotic functionality from arabinan would represent significant added value to a renewable resource.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13584
Uncontrolled Keywords:arabinan, arabino-oligosaccharides, fluorescence in situ hybridization, prebiotics sugar beet, RICH PECTIC POLYSACCHARIDES, LACTIC-ACID BACTERIA, OLIGONUCLEOTIDE, PROBES, SITU HYBRIDIZATION, CONTINUOUS-CULTURE, BACTEROIDES, BIFIDOBACTERIUM, DIGESTION, GROWTH, PULP

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation