Analysis of growth physiology and phytochemical content of Eruca and Diplotaxis Cultivars under different light and temperature regimes
Wagstaff, C. (2014) Analysis of growth physiology and phytochemical content of Eruca and Diplotaxis Cultivars under different light and temperature regimes. Acta Horticulturae, 1040. pp. 361-374. ISSN 0567-7572
Official URL: http://www.actahort.org/books/1040/1040_49.htm
Rocket is a leafy brassicaceous salad crop that encompasses two major genera (Diplotaxis and Eruca) and many different cultivars. Rocket is a rich source of antioxidants and glucosinolates, many of which are produced as secondary products by the plant in response to stress. In this paper we examined the impact of temperature and light stress on several different cultivars of wild and salad rocket. Growth habit of the plants varied in response to stress and with different genotypes, reflecting the wide geographical distribution of the plant and the different environments to which the genera have naturally adapted. Preharvest environmental stress and genotype also had an impact on how well the cultivar was able to resist postharvest senescence, indicating that breeding or selection of senescence-resistant genotypes will be possible in the future. The abundance of key phytonutrients such as carotenoids and glucosinolates are also under genetic control. As genetic resources improve for rocket it will therefore be possible to develop a molecular breeding programme specifically targeted at improving stress resistance and nutritional levels of plant secondary products. Concomitantly, it has been shown in this paper that controlled levels of abiotic stress can potentially improve the levels of chlorophyll, carotenoids and antioxidant activity in this leafy vegetable.
Bennett, R.N., Rosa, E.A., Mellon, F.A. and Kroon, P.A. 2006. Ontogenic profiling of glucosinolates, flavonoids, and other secondary metabolites in Eruca sativa (salad rocket), Diplotaxis erucoides (wall rocket), Diplotaxis tenuifolia (wild rocket), and Bunias orientalis (Turkish rocket). J. Agric. Food Chem. 54: 4005-4015. Benzie, I.F.F. and Strain, J.J. 1969. The Ferric Reducing Ability of Plasma (FRAP) as a Measurement of “Antioxidant Power”: The FRAP Assay. Analytical Biochem. 239: 70-76. Chenna, R., Sugawara, H., Koike,T., Lopez, R., Gibson, T.J., Higgins, D.G. and Thompson, J.D. Multiple sequence alignment with the Clustal series of programs. 2003. Nucl. Acids Res. 31: 3497-3500. Galmes, J., Abadi, A., Cifrea, J., Medranoa, H. and Flexas, J. 2007. Photoprotection processes under water stress and recovery in Mediterranean plants with different growth forms and leaf habits. Physiol. Plant. 130: 495-510. Gosslau, A. and Chen, K.Y. 2004. Nutraceuticals, apoptosis, and disease prevention. J. Nutrition 20: 95-102. Gundgaard, J., Nielsen, J. N., Olsen, J. and Sorensen, J. 2003. Increased intake of fruit and vegetables: Estimation of impact in terms of life expectancy and healthcare costs. Public Health Nutr. 6: 25-30. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser. 41: 95-98. Higdon, J.V., Delage, B., Williams, D.E. and Dashwood, R.H. 2007. Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basis. Pharmacol. Res. 55: 224-236. Kentaro, M., Sumiko, S. and Hiroshi, G. 2005. Decreased anthocyanin biosynthesis in grape berries grown under elevated night temperature condition. Sci. Hort. 105: 319-330. Kim, S-J. and Ishii, G. 2007. Effect of storage temperature and duration on glucosinolate, total vitamin C and nitrate contents in rocket salad (Eruca sativa Mill.) J. Sci. Food Agric. 87: 966-973. Kliebenstein, D.J., Kroymann, J., Brown, P., Figuth, A., Pedersen, D., Gershenzon, J. and Mitchell-Olds, T. 2001. Genetic control of natural variation in Arabidopsis glucosinolate accumulation. Plant Physiol. 126: 811-825. Law, M.R. and Morris, J.K. 1998. By how much does fruit and vegetable consumption reduce the risk of ischemic heart disease? Euro. J. Clin. Nutr. 52: 549-556. Leja, M., Mareczek, A., Starzynska, A. and Rozek, S. 2001. Antioxidant ability of broccoli flower buds during short-term storage. Food Chem. 72: 219-222. Leyva, A., Jarillo, J.A., Salinas, J. and Martinez-Zapater, J.M. 1995. Low temperature induces the accumulation of phenylalanine ammonia-lyase and chalcone synthase mRNAs of Arabidopsis thaliana in a light-dependent manner. Plant Physiology, 108: 39-46. Lindley, M.G. 1998. The impact of food processing on antioxidants in vegetable oils, fruits and vegetables. Trends Food Sci. Technol. 9: 336-340. Martinez-Sanchez, A., Llorach, R., Gil, M.I. and Ferreres, F. 2007. Identification of new flavonoid glycosides and flavonoid profiles to characterize rocket leafy salads (Eruca vesicaria and Diplotaxis tenuifolia). J. Agric. Food Chem. 55: 1356-1363. Martinez-Sanchez, A., Marın, A., Llorach, R., Ferreres, F. and Gil, M.I. 2006. Controlled atmosphere preserves quality and phytonutrients in wild rocket (Diplotaxis tenuifolia). Postharvest Biol. Technol. 40: 26-33. Simmonds, M.S. 2003. Flavonoid-insect interactions: recent advances in our knowledge. Phytochem. 64: 21-30. Verkerk, R., M.C. Schreiner, A. Krumbein, E. Ciska, B. Holst, I. Rowland, R. De Schrijver, M. Hansen, C. Gerhäuser, R. Mithen and M. Dekker, 2009. Glucosinolates from Brassica vegetables; The influence of the food supply chain on intake, bioavailability and human health, Molecular Nutrition and Food Research, 53, S219 –S265 VVAA. 1997. Rocket: A Mediterranean Crop for the World. In: Padulosi, S. and Pignone, D. (Eds.), International Plant Genetic Resources Institute: Rome, Italy. Wagstaff, C., Leverentz, M., Griffiths, G., Thomas, B., Chanasut, U., Stead, A. and Rogers, H. 2002. Cysteine protease gene expression and proteolytic activity during senescence of Alstroemeria petals. J. Exptl. Bot. 53: 233–240. Wellburn, A.R. 1994. The spectral determination of chlorophyll-a and chlorophyll-b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Plant Physiol. 144: 307-313. Winkler, S., Faraghera, J., Franza, P., Imsica, M. and Jones, R. 2007. Glucoraphanin and flavonoid levels remain stable during simulated transport and marketing of broccoli (Brassica oleracea var. italica) heads. Postharvest Biol. Technol. 43: 89-94. Zhang, Z., Ober, J.A. and Kliebenstein, D.J. 2006. The gene controlling the quantitative trait locus EPITHIOSPECIFIER MODIFIER1 alters glucosinolate hydrolysis and insect resistance in Arabidopsis. Plant Cell 18: 1524-1536.