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Characterization of virus-like particles associated with the human faecal and caecal microbiota

Hoyles, L., McCartney, A. L., Neve, H., Gibson, G. R., Sanderson, J. D., Heller, K. J. and van Sinderen, D. (2014) Characterization of virus-like particles associated with the human faecal and caecal microbiota. Research in Microbiology, 165 (10). pp. 803-812. ISSN 0923-2508

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To link to this item DOI: 10.1016/j.resmic.2014.10.006

Abstract/Summary

This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group
ID Code:37814
Uncontrolled Keywords:Bacteriophages; Microbial ecology; Gastrointestinal tract; Transmission electron microscopy; Caecum; Faeces
Publisher:Elsevier

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