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Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health

Brown, E. M., Nitecki, S., Pereira-Caro, G., McDougall, G. J., Stewart, D., Rowland, I., Crozier, A. and Gill, C. I. R. (2014) Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health. BioFactors, 40 (6). pp. 611-623. ISSN 1872-8081

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To link to this item DOI: 10.1002/biof.1173

Abstract/Summary

The composition of polyphenols in ileal fluid samples obtained from an ileostomy subject after lingonberry intake was compared with lingonberry extracts obtained after simulated in vitro digestion (IVDL) and subsequent faecal fermentation (IVFL). HPLC-PDA-MS/MS analysis confirmed similar patterns of lingonberry (poly)phenolic metabolism after the in vivo and in vitro digestion, with reduced recovery of anthocyanins and a similar pattern of recovery for proanthocyanidins observed for both methods of digestion. On the other hand, the IVFL sample contained none of the original (poly)phenolic components but was enriched in simple aromatic components. Digested and fermented extracts exhibited significant (P < 0.05) anti-genotoxic (Comet assay), anti-mutagenic (Mutation Frequency assay), and anti-invasive (Matrigel Invasion assay) effects in human cell culture models of colorectal cancer at physiologically-relevant doses (0-50 μg/mL gallic acid equivalents). The ileal fluid induced significant anti-genotoxic activity (P < 0.05), but at a higher concentration (200 μg/mL gallic acid equivalents) than the IVDL. Despite extensive structural modification following digestion and fermentation, lingonberry extracts retained their bioactivity in vitro. This reinforces the need for studies to consider the impact of digestion when investigating bioactivity of dietary phytochemicals.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group
ID Code:38214
Uncontrolled Keywords:(poly)phenol; lingonberry; colorectal cancer; digestion; ileostomy; anti-genotoxic
Publisher:Wiley

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