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Staphylococcus aureus MnhF mediates cholate efflux and facilitates survival under human colonic conditions

Sannasiddappa, T., Hood, G., Hanson, K., Costabile, A., Gibson, G. and Clarke, S. (2015) Staphylococcus aureus MnhF mediates cholate efflux and facilitates survival under human colonic conditions. Infection and Immunity, 83 (6). pp. 2350-2357. ISSN 0019-9567

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To link to this item DOI: 10.1128/IAI.00238-15

Abstract/Summary

Resistance to the innate defences of the intestine is crucial for the survival and carriage of Staphylococcus aureus, a common coloniser of the human gut. Bile salts produced by the liver and secreted into the intestines are one such group of molecules with potent anti-microbial activity. The mechanisms by which S. aureus is able to resist such defences in order to colonize and survive in the human gut are unknown. Here we show that mnhF confers resistance to bile salts, which can be abrogated by efflux pump inhibitors. MnhF mediates efflux of radiolabelled cholic acid in both S. aureus and when heterologously expressed in Escherichia coli, rendering them resistant. Deletion of mnhF attenuated survival of S. aureus in an anaerobic three stage continuous culture model of the human colon (gut model), which represent different anatomical areas of the large intestine.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences > Biomedical Sciences
Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group
ID Code:39959
Publisher:American Society for Microbiology

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