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Development of a pipeline for exploratory metabolic profiling of infant urine

Jackson, F., Georgakopoulou, N., Kaluarachchi, M., Kyriakides, M., Andreas, N., Przysiezna, N., Hyde, M. J., Modi, N., Nicholson, J. K., Wijeyesekera, A. and Holmes, E. (2016) Development of a pipeline for exploratory metabolic profiling of infant urine. Journal of Proteome Research, 15 (9). pp. 3432-3440. ISSN 1535-3907

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To link to this item DOI: 10.1021/acs.jproteome.6b00234

Abstract/Summary

Numerous metabolic profiling pipelines have been developed to characterize the composition of human biofluids and tissues, the vast majority of these being for studies in adults. To accommodate limited sample volume and to take into account the compositional differences between adult and infant biofluids, we developed and optimized sample handling and analytical procedures for studying urine from newborns. A robust pipeline for metabolic profiling using NMR spectroscopy was established, encompassing sample collection, preparation, spectroscopic measurement, and computational analysis. Longitudinal samples were collected from five infants from birth until 14 months of age. Methods of extraction and effects of freezing and sample dilution were assessed, and urinary contaminants from breakdown of polymers in a range of diapers and cotton wool balls were identified and compared, including propylene glycol, acrylic acid, and tert-butanol. Finally, assessment of urinary profiles obtained over the first few weeks of life revealed a dramatic change in composition, with concentrations of phenols, amino acids, and betaine altering systematically over the first few months of life. Therefore, neonatal samples require more stringent standardization of experimental design, sample handling, and analysis compared to that of adult samples to accommodate the variability and limited sample volume.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group
ID Code:83323
Publisher:American Chemical Society

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