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Enzymatic hydrolysis of thermally pre‐treated chitin and antimicrobial activity of N,N'‐diacetylchitobiose

Zainal Abidin, M., Kourmentza, C., Karatzas, K. A. and Niranjan, K. (2019) Enzymatic hydrolysis of thermally pre‐treated chitin and antimicrobial activity of N,N'‐diacetylchitobiose. Journal of Chemical Technology and Biotechnology. ISSN 1097-4660 (In Press)

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To link to this item DOI: 10.1002/jctb.6043

Abstract/Summary

BACKGROUND: N,N’-diacetylchitobiose (GlcNAc2) is known to be highly functional and offers a wide range of applications, especially as an antimicrobial agent. In this study, a thermal pre-treatment process using steam under pressure in an autoclave, has been employed to facilitate subsequent enzymatic hydrolysis of chitin with chitinase from Streptomyces griseus. RESULTS: Pre-treatment of chitin with 0.05 M sodium acetate buffer (pH=6.0) at 121ºC for 60 min, followed by enzymatic hydrolysis involving 24 h incubation, was found to be the best condition for producing the GlcNAc2. The GlcNAc2 obtained was tested regarding its antimicrobial activity against Gram-negative and Gram-positive strains and showed minimum inhibitory concentrations (MIC) at 5 and 10% w/v against Escherichia coli K-12 and Listeria monocytogenes 10403S, respectively. CONCLUSION: The extent of swelling and crystallite size of chitin increased with the pre-treatment residence time, and enhanced the rate of subsequent hydrolysis using chitinase.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group
Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Research Group
ID Code:83411
Publisher:Wiley

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