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Thiolytic screening method for exploring condensed tannin variation in a unique sainfoin germplasm bank

Stringano, E., Hayot, C., Smith, L., Theodoridou, K., Aufrere, J., Brown, R., Hayes, W. ORCID:, Cramer, R. ORCID: and Mueller-Harvey, I. (2010) Thiolytic screening method for exploring condensed tannin variation in a unique sainfoin germplasm bank. Planta Medica, 76 (12). 0_3. ISSN 1439-0221

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To link to this item DOI: 10.1055/s-0030-1264189


This EU funded 'HealthyHay'project stablished a sainfoin (Onobrychis vicifolia) germplasm bank at NIAB, Cambridge, with 306 accessions from around the world. A screening method was developed to characterise tannins by thiolytic degradation [1] directly in green plants for the first time. the method was validated by separate analysis of unextractable, extractable and purified tannins using thiolysis, HPLC-GPC and MALDI-TOF MS. Most tannins (58 to 73% of the total) could be recovered after Toyopearl HW50 fractionation with water, aqueous methanol and acetone. the greatest losses during purification occurred amongst larger molecular weight tannins with mean degree of polymerisation (mDP) > 18. The composition of water-,aqueous methanol- and acetone-soluble tannins differed considerably in their mDP and trans/cis ratios, but not in their prodelphinidin/orocyanidin (PD/PC) ratios.

Item Type:Article
Divisions:Life Sciences
Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Interdisciplinary centres and themes > Chemical Analysis Facility (CAF)
ID Code:18405
Additional Information:Special issue containing the proceedings of the 7th Tannin Conference (Presymposium) and 58th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research: Berlin, Germany 29th August - 2nd September 2010
Publisher:Thieme Medical Publishers, Inc.

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