Accessibility navigation


Synthesis of 3 '-S-phosphorothiolate oligonucleotides for their potential use in RNA interference

Gaynor, J. W., Brazier, J. ORCID: https://orcid.org/0000-0002-4952-584X and Cosstick, R. (2007) Synthesis of 3 '-S-phosphorothiolate oligonucleotides for their potential use in RNA interference. Nucleosides Nucleotides & Nucleic Acids, 26 (6-7). pp. 709-712. ISSN 1525-7770

Full text not archived in this repository.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1080/15257770701490753

Abstract/Summary

The potency of RNA interference (RNAi) undoubtedly can be improved through chemical modifications to the small interfering RNAs (siRNA). By incorporation of the 3′-S-phosphorothiolate modification into strands of RNA, it is hoped that specific regions of a siRNA duplex can be stabilised to enhance the target binding affinity of a selected antisense strand into the activated RNA-induced silencing complex (RISC*). Oligonucleotides composed entirely of this modification are desirable so unconventional 5′ → 3′ synthesis is investigated, with initial solution-phase testing proving successful. The phosphoroamidite monomer required for solid-phase synthesis has also been produced.

Item Type:Article
Refereed:Yes
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Medicinal Chemistry Research Group
ID Code:27314
Uncontrolled Keywords:RNA Interference, siRNA, phosphorothiolate, reverse synthesis, 5′-3′
Additional Information:Presented at the Joint Symposium of 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids (IRTXVIII) and 35th International Symposium on Nucleic Acids Chemistry (SNAC)Times Cited: 3 Proceedings of the 17th International Roundtable of Nucleosides, Nucleotides, and Nucleic Acids MAR 07-SEP 07, 2006 Bern, SWITZERLAND

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation