Analysis of extracellular vesicles using fluorescence nanoparticle tracking analysisCarnell-Morris, P., Tannetta, D., Siupa, A., Hole, P. and Dragovic, R. (2017) Analysis of extracellular vesicles using fluorescence nanoparticle tracking analysis. In: Extracellular Vesicles. Springer, pp. 153-173. ISBN 9781493972531 Full text not archived in this repository. It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing. To link to this item DOI: 10.1007/978-1-4939-7253-1_13 Abstract/SummaryFluorescence nanoparticle tracking analysis (fl-NTA) allows for accurate sizing, counting, and phenotyping of extracellular vesicles (EV). Here, we present two protocols for the analysis of EVs using fl-NTA, highlighting the potential pitfalls and challenges. The first protocol utilizes CellMask Orange™ (CMO) as a general membrane marker to label EVs derived from plasma. The second protocol describes the use of a Qdot-conjugated antibody to identify syncytiotrophoblast (STB)-derived EVs. "Standard" preparations of STB-derived EVs enriched for either microvesicles (STBMV) or exosomes (STBEX), containing a known amount of EV positive for the STB specific antigen placental alkaline phosphatase (PLAP), were also used to optimize fl-NTA camera settings.
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