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In vitro fermentation of copra meal hydrolysate by human fecal microbiota

Prayoonthien, P., Rastall, R. A., Kolida, S., Nitisinprasert, S. and Keawsompong, S. (2019) In vitro fermentation of copra meal hydrolysate by human fecal microbiota. 3 Biotech, 9. 93. ISSN 2190-5738

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To link to this item DOI: 10.1007/s13205-019-1633-8


Copra meal hydrolysate (CMH) is obtained by hydrolyzing defatted copra meal with β-mannanase from NT 6.7. In this study, we investigated the resistance of CMH to upper gastrointestinal tract digestion and the fecal fermentation profiles of CMH. Fecal slurries from four healthy human donors were used as inocula, and fructooligosaccharides (FOS) were used as a positive prebiotic control. Fecal batch cultures were performed at 37 °C under anaerobic conditions. Samples were collected at 0, 10, 24 and 34 h for bacterial enumeration via fluorescent in situ hybridization and organic acid (OA) analysis. In vitro gastric stomach and human pancreatic α-amylase simulations demonstrated that CMH was highly resistant to hydrolysis. Acetate was the main fermentation product of all the substrates. The proportions of acetate production of the total OAs from FOS, CMH and yeast mannooligosaccharides (MOS) after 34 h of fermentation did not significantly differ (69.76, 65.24 and 53.93%, respectively). At 24 h of fermentation, CMH promoted the growth of and groups (  < 0.01) and did not significantly differ from the results obtained using FOS. The results of in vitro fecal fermentation of CMH indicate that CMH can promote the growth of beneficial bacteria.

Item Type:Article
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Research Group
ID Code:82713
Uncontrolled Keywords:Coconut, Copra meal hydrolysate, Human fecal microbiota, In vitro fermentation

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