Accessibility navigation

Infusion of donor feces affects the gut–brain axis in humans with metabolic syndrome

Hartstra, A. V., Schüppel, V., Imangaliyev, S., Schrantee, A., Prodan, A., Collard, D., Levin, E., Dallinga-Thie, G., Ackermans, M. T., Winkelmeijer, M., Havik, S. R., Metwaly, A. ORCID:, Lagkouvardos, I., Nier, A., Bergheim, I., Heikenwalder, M., Dunkel, A., Nederveen, A. J., Liebisch, G., Mancano, G. , Claus, S. P., Benítez-Páez, A., la Fleur, S. E., Bergman, J. J., Gerdes, V., Sanz, Y., Booij, J., Kemper, E., Groen, A. K., Serlie, M. J., Haller, D. and Nieuwdorp, M. (2020) Infusion of donor feces affects the gut–brain axis in humans with metabolic syndrome. Molecular Metabolism, 42. 101076. ISSN 22128778

Text (Open Access) - Published Version
· Available under License Creative Commons Attribution.
· Please see our End User Agreement before downloading.


It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1016/j.molmet.2020.101076


Objective Increasing evidence indicates that intestinal microbiota play a role in diverse metabolic processes via intestinal butyrate production. Human bariatric surgery data suggest that the gut-brain axis is also involved in this process, but the underlying mechanisms remain unknown. Methods We compared the effect of fecal microbiota transfer (FMT) from post-Roux-en-Y gastric bypass (RYGB) donors vs oral butyrate supplementation on (123I-FP-CIT-determined) brain dopamine transporter (DAT) and serotonin transporter (SERT) binding as well as stable isotope-determined insulin sensitivity at baseline and after 4 weeks in 24 male and female treatment-naïve metabolic syndrome subjects. Plasma metabolites and fecal microbiota were also determined at these time points. Results We observed an increase in brain DAT after donor FMT compared to oral butyrate that reduced this binding. However, no effect on body weight and insulin sensitivity was demonstrated after post-RYGB donor feces transfer in humans with metabolic syndrome. Increases in fecal levels of Bacteroides uniformis were significantly associated with an increase in DAT, whereas increases in Prevotella spp. showed an inverse association. Changes in the plasma metabolites glycine, betaine, methionine, and lysine (associated with the S-adenosylmethionine cycle) were also associated with altered striatal DAT expression. Conclusions Although more and larger studies are needed, our data suggest a potential gut microbiota-driven modulation of brain dopamine and serotonin transporters in human subjects with obese metabolic syndrome. These data also suggest the presence of a gut-brain axis in humans that can be modulated.

Item Type:Article
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Research Group
ID Code:93359


Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation