Convergent hemostatic disruption by distinct venom architectures in Bothrops spp. and Daboia russelii

Malchias-Pires, G. M., Romanazzi, M., Almeida, J. R., Williams, J., Vijayakumar, P., Gilabadi, S., Junior, R. S. F., Vaiyapuri, S. ORCID: https://orcid.org/0000-0002-6006-6517 and Pucca, M. B. (2026) Convergent hemostatic disruption by distinct venom architectures in Bothrops spp. and Daboia russelii. Biochemistry Research International. ISSN 2090-2255 (In Press)

Abstract/Summary

Snake venoms from the genera Bothrops and Daboia are well known for inducing severe hemostatic disturbances and are responsible for significant clinical manifestations associated with snakebite envenomation in different regions worldwide. Although these venoms share similar toxin families, such as metalloproteinases, serine proteases, and phospholipases A₂, they exhibit biochemical and functional differences that result in distinct coagulopathic mechanisms. In this study, an integrated comparative analysis of venoms from different Bothrops species and Daboia russelii was performed using and functional approaches to elucidate their effects on the human coagulation system. Venoms were characterized by SDS-PAGE and enzymatic assays to assess metalloproteinase, serine protease, and PLA₂ activities, as well as fibrinogenolytic activity. Hemostatic effects were investigated using rotational thromboelastometry (ROTEM), prothrombin time (PT), and activated partial thromboplastin time (aPTT). The results demonstrated that, although both genera are capable of inducing venom-induced consumption coagulopathy, Bothrops venoms display a more heterogeneous profile strongly associated with fibrinogen degradation and clot instability, whereas D. russelii exhibits a more targeted procoagulant effect linked to the specific activation of coagulation cascade factors. These findings highlight the complexity of venom-induced coagulopathies and emphasize the importance of comparative approaches for improving therapeutic strategies and the development of more effective antivenoms.

Item Type	Article
URI	https://centaur.reading.ac.uk/id/eprint/130474
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
Publisher	Wiley
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