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Loss of 3-chlorotyrosine by inflammatory oxidants: Implications for the use of 3-chlorotyrosine as a bio-marker in vivo

Whiteman, M. and Spencer, J. P. E. (2008) Loss of 3-chlorotyrosine by inflammatory oxidants: Implications for the use of 3-chlorotyrosine as a bio-marker in vivo. Biochemical and Biophysical Research Communications, 371 (1). pp. 50-53. ISSN 0006-291X

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To link to this article DOI: 10.1016/j.bbrc.2008.03.153

Abstract/Summary

Activated neutrophils generate the potent oxidant hypochlorous acid (HOCl) from the enzyme myeloperoxidase (MPO). A proposed bio-marker for MPO-derived HOCl in vivo is 3-chlorotyrosine, elevated levels of which have been measured in several human inflammatory pathologies. However, it is unlikely that HOCl is produced as the sole oxidant at sites of chronic inflammation as other reactive species are also produced during the inflammatory response. The work presented shows that free and protein bound 3-chlorotyrosine is lost upon addition of the pro-inflammatory oxidants, HOCl, peroxynitrite, and acidified nitrite. Furthermore, incubation of 3-chlorotyrosine with activated RAW264.7 macrophages or neutrophil-like HL-60 cells resulted in significant loss of 3-chlorotyrosine. Therefore, at sites of chronic inflammation where there is concomitant ONOO- and HOCl formation, it is possible measurement of 3-chlorotyrosine may represent an underestimate of the true extent of tyrosine chlorination. This finding could account for some of the discrepancies reported between 3-chlorotyrosine levels in tissues in the literature. (c) 2008 Elsevier Inc. All rights reserved.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
ID Code:12858
Uncontrolled Keywords:peroxynitrite, hypochlorous acid, reactive chlorine species, 3-chlorotyrosine, inflammation, CALF THYMUS DNA, HYPOCHLOROUS ACID, NITRIC-OXIDE, CYSTIC-FIBROSIS, OBSTRUCTIVE CHOLESTASIS, RHEUMATOID-ARTHRITIS, ALZHEIMERS-DISEASE, PROTEIN OXIDATION, HUMAN-NEUTROPHILS, TYROSYL RESIDUES

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