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Differences in cell morphology, lipid and apo B secretory capacity in caco-2 cells following long term treatment with saturated and monounsaturated fatty acids

Bateman, P.A., Jackson, K.G., Maitin, V., Yaqoob, P. and Williams, C.A. (2007) Differences in cell morphology, lipid and apo B secretory capacity in caco-2 cells following long term treatment with saturated and monounsaturated fatty acids. Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids, 1771 (4). pp. 475-485. ISSN 1388-1981

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To link to this article DOI: 10.1016/j.bbalip.2007.02.001

Abstract/Summary

The suitability of the caco-2 cell line as a model for studying the long term impact of dietary fatty acids on intestinal lipid handling and chylomicron production was examined. Chronic supplementation of caco-2 cells with palmitic acid (PA) resulted in a lower triacylglycerol secretion than oleic acid (OA). This was coupled with a detrimental effect of PA, but not OA, on transepithelial electrical resistance (TER) measurements, suggesting a loss of structural integrity across the cell monolayer. Addition of OA reversed the adverse effects of PA and stearic acid on TER and increased the ability of cells to synthesise and accumulate lipid, but did not normalise the secretion of lipids by caco-2 cells. Increasing amounts of OA and decreasing amounts of PA in the incubation media markedly improved the ability of cells to synthesise apolipoprotein B and secrete lipids. Real time RT-PCR revealed a down regulation of genes involved in lipoprotein synthesis following PA than OA. Electron microscopy showed adverse effects of PA on cellular morphology consistent with immature enterocytes such as stunted microvilli and poor tight junction formation. In conclusion, previously reported differences in lipoprotein secretion by caco-2 cells supplemented with saturated fatty acids (SFA) and OA may partly reflect early cytotoxic effects of SFA on cellular integrity and function. (C) 2007 Elsevier B.V. All rights reserved.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
ID Code:13566
Uncontrolled Keywords:apolipoprotein B, monounsaturated fatty acid, saturated fatty acid, real time RT-PCR, taurocholate micelle, tight junction, ISOLATED RABBIT ENTEROCYTES, LIPOPROTEIN SECRETION, APOLIPOPROTEIN-B, FISH OIL, RICH LIPOPROTEINS, GENE-EXPRESSION, CHAIN-LENGTH, OLEIC-ACID, METABOLISM, CHYLOMICRONS

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