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mTOR-related neuropathology in mutant tsc2 zebrafish: Phenotypic, transcriptomic and pharmacological analysis

Scheldeman, C., Mills, J. D., Siekierska, A., Serra, I., Copmans, D., Iyer, A. M., Whalley, B. J., Maes, J., Jansen, A. C., Lagae, L., Aronica, E. and de Witte, P. A. M. (2017) mTOR-related neuropathology in mutant tsc2 zebrafish: Phenotypic, transcriptomic and pharmacological analysis. Neurobiology of disease, 108. pp. 225-237. ISSN 1095-953X

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To link to this item DOI: 10.1016/j.nbd.2017.09.004

Abstract/Summary

Tuberous sclerosis complex (TSC) is a rare, genetic disease caused by loss-of-function mutations in either TSC1 or TSC2. Patients with TSC are neurologically characterized by the presence of abnormal brain structure, intractable epilepsy and TSC-associated neuropsychiatric disorders. Given the lack of effective long-term treatments for TSC, there is a need to gain greater insight into TSC-related pathophysiology and to identify and develop new treatments. In this work we show that homozygous tsc2(-/-) mutant zebrafish larvae, but not tsc2(+/-) and WT larvae, display enlarged brains, reduced locomotor behavior and epileptiform discharges at 7dpf. In addition, we pharmacologically validated the TSC model by demonstrating the dramatic rescue effect of pericardially injected rapamycin, a well-known mTOR inhibitor, on selected behavioral read-outs and at the molecular level. By means of trancriptome profiling we also acquired more insight into the neuropathology of TSC, and as a result were able to highlight possible new treatment targets. The gene expression profiles of WT and tsc2(+/-) larvae revealed 117 differentially expressed genes (DEGs), while between WT and tsc2(-/-) larvae and tsc2(+/-) and tsc2(-/-) larvae there were 1414 and 1079 DEGs, respectively. Pathway enrichment analysis from the WT and tsc2(-/-) DEGs, identified 14 enriched pathways from the up-regulated genes and 6 enriched pathways from the down-regulated genes. Moreover, genes related to inflammation and immune response were up-regulated in the heads of tsc2(-/-) larvae, in line with the findings in human brain tissue where inflammatory and immune responses appear to be major hallmarks of TSC. Taken together, our phenotypic, transcriptomic and pharmacological analysis identified the tsc2(-/-) zebrafish as a preclinical model that mirrors well aspects of the human condition and delineated relevant TSC-related biological pathways. The model may be of value for future TSC-related drug discovery and development programs. [Abstract copyright: Copyright © 2017. Published by Elsevier Inc.]

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Psychology and Clinical Language Sciences > Department of Psychology
Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
ID Code:72589
Uncontrolled Keywords:Transcriptomics, Tuberous sclerosis complex, Zebrafish, mTOR
Publisher:Elsevier

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