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In vitro antioxidant activity of coffee compounds and their metabolites

Gomez-Ruiz, J. A., Leake, D. S. and Ames, J. M. (2007) In vitro antioxidant activity of coffee compounds and their metabolites. Journal of Agricultural and Food Chemistry, 55 (17). pp. 6962-6969. ISSN 0021-8561

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To link to this article DOI: 10.1021/jf0710985

Abstract/Summary

In this paper we report the antioxidant activity of different compounds which are present in coffee or are produced as a result of the metabolism of this beverage. In vitro methods such as the ABTS(center dot+) [ABTS = 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] decolorization assay and the oxygen radical absorbance capacity assay (ORAC) were used to assess the capacity of coffee compounds to scavenge free radicals. The importance of caffeine metabolites and colonic metabolites in the overall antioxidant activity associated with coffee consumption is shown. Colonic metabolites such as m-coumaric acid and dihydroferulic acid showed high antioxidant activity. The ability of these compounds to protect human low-density lipoprotein (LDL) oxidation by copper and 2,2'-azobis(2-amidinopropane) dihydrochloride was also explored. 1-Methyluric acid was particularly effective at inhibiting LDL oxidative modification. Different experiments showed that this caffeine metabolite is not incorporated into LDL particles. However, at physiologically relevant concentrations, it was able to delay for more than 13 h LDL oxidation by copper.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
Faculty of Life Sciences > School of Biological Sciences
Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
ID Code:9994
Uncontrolled Keywords:coffee, antioxidant activity, LDL, ORAC, ABTS, coffee metabolites, CAFFEIC ACID, CHLOROGENIC ACID, HUMANS, VIVO, DERIVATIVES, LDL, BIOAVAILABILITY, CONSUMPTION, INHIBITION, ABSORPTION
Publisher:American Chemical Society

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