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Interaction of flavonoids with bovine serum albumin: a fluorescence quenching study

Papadopoulou, A., Green, R. J. and Frazier, R. A. ORCID: (2005) Interaction of flavonoids with bovine serum albumin: a fluorescence quenching study. Journal of Agricultural and Food Chemistry , 53 (1). pp. 158-163. ISSN 0021-8561

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To link to this item DOI: 10.1021/jf048693g


The interaction between four flavonoids (catechin, epicatechin, rutin and quercetin) and bovine serum albumin (BSA) was investigated using tryptophan fluorescence quenching. Quenching constants were determined using the Stern-Volmer equation to provide a measure of the binding affinity between the flavonoids and BSA. The binding affinity was found to be strongest for quercetin, and ranked in the order quercetin>rutin>epicatechin=catechin. The pH in the range of 5 to 7.4 does not affect significantly (p<0.05) the association of rutin, epicatechin and catechin with BSA, but quercetin exhibited a stronger affinity at pH 7.4 than at lower pH (p<0.05). Quercetin has a total quenching effect on BSA tryptophan fluorescence at a molar ratio of 10:1 and rutin at approximately 25:1. However, epicatechin and catechin did not fully quench tryptophan fluorescence over the concentration range studied. Furthermore, the data suggested that the association between flavonoids and BSA did not change molecular conformation of BSA and that hydrogen bonding, ionic and hydrophobic interaction are equally important driving forces for protein-flavonoid association.

Item Type:Article
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Pharmaceutics Research Group
Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Research Group
ID Code:7930
Uncontrolled Keywords:flavonoid, polyphenol, protein, albumin, tryptophan fluorescence, quenching, spectroscopy, TANNIN-PROTEIN COMPLEXES, TRYPTOPHAN FLUORESCENCE, ANTIOXIDANT, ACTIVITY, BINDING-SITES, HEART-DISEASE, METABOLISM, POLYPHENOLS, CHEMISTRY, ABSORPTION, CANCER
Publisher:American Chemical Society

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