Accessibility navigation


In vitro fermentation of carbohydrates by porcine faecal inocula and their influence on Salmonella Typhimurium growth in batch culture systems

Martin-Pelaez, S., Gibson, G. R., Martin-Orue, S. M., Klinder, A., Rastall, R. A., La Ragione, R. M., Woodward, M. J. and Costabile, A. (2008) In vitro fermentation of carbohydrates by porcine faecal inocula and their influence on Salmonella Typhimurium growth in batch culture systems. Fems Microbiology Ecology, 66 (3). pp. 608-619. ISSN 0168-6496

Full text not archived in this repository.

To link to this article DOI: 10.1111/j.1574-6941.2008.00610.x

Abstract/Summary

The aim of this study was to evaluate in vitro the influence of fermentable carbohydrates on the activity of porcine microbiota and survival of Salmonella Typhimurium in a batch culture system simulating the porcine hindgut. The carbohydrates tested were xylooligosaccharides, a mixture of fructooligosaccharides/inulin (FIN), fructooligosaccharides (FOS), gentiooligosaccharides (GEO) and lactulose (LAC). These ingredients stimulated the growth of selected Bifidobacterium and Lactobacillus species in pure cultures. In batch cultures, the carbohydrates influenced some fermentation parameters. For example, GEO and FIN significantly increased lactic acids compared with the control (no added carbohydrate). With the exception of LAC, the test carbohydrates increased the production of short-chain fatty acid (SCFA) and modified SCFA profiles. Quantitative analysis of bacterial populations by FISH revealed increased counts of the Bifidobacterium group compared with control and, with exception of FOS, increased Lactobacillus, Leuconostoc and Weissella spp. counts. Salmonella numbers were the lowest during the fermentation of LAC. This work has looked at carbohydrate metabolism by porcine microbiota in a pH-controlled batch fermentation system. It provides an initial model to analyse interactions with pathogens.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13161
Uncontrolled Keywords:batch cultures, pigs, nondigestible carbohydrates, Salmonella, Typhimurium, short-chain fatty acids, FISH, LACTIC-ACID BACTERIA, CHAIN FATTY-ACIDS, ENTERICA SEROVAR TYPHIMURIUM, RNA-BASED PROBES, RIBOSOMAL-RNA, HUMAN FECES, OLIGONUCLEOTIDE PROBES, INTESTINAL MICROBIOTA, SITU HYBRIDIZATION, GENE-EXPRESSION

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation