Preparation of (–)-epigallocatechin gallate from commercial green tea by caffeine precipitation and solvent partition
Copeland, E. L., Clifford, M. N. and Williams, C. M. (1998) Preparation of (–)-epigallocatechin gallate from commercial green tea by caffeine precipitation and solvent partition. Food Chemistry, 61 (1-2). pp. 81-87. ISSN 0308-8146
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To link to this article DOI: 10.1016/S0308-8146(97)00111-8
A method has been developed which enables the easy and inexpensive preparation of gram quantities of (–)-epigallocatechin gallate from green tea (Camellia sinensis). A decaffeinated aqueous brew of commercial green tea is treated with caffeine (30 m ). The precipitate is redissolved after decaffeination with chloroform and further purified by solvent partition with ethyl hexanoate and propyl acetate. Commercial leaf (25 g) yields 400 mg (–)-epigallocatechin gallate at better than 80% purity, as judged by reversed phase HPLC.