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Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation

Zaini, N. A., Chatzifragkou, A., Tverezovskiy, V. and Charalampopoulos, D. (2019) Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation. Biochemical Engineering Journal. ISSN 1369-703X (In Press)

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To link to this item DOI: 10.1016/j.bej.2019.107265

Abstract/Summary

A multi-step process was developed for microbial D-lactic acid purification, followed by poly-D-lactic acid (PDLA) synthesis via azeotropic polycondensation process. Several anion exchange resins were screened for their binding capacity using model lactic acid solutions. Amberlite® IRA67 (weak base anion exchange resin) showed the highest lactic acid adsorption, with maximum adsorption capacity, qmax, of 136.11 mg lactic acid / g of resin, and was further selected to purify D-lactic acid from DDGS hydrolysates through a three-step process; (1) treatment with 7% w/v activated carbon, (2) acidification of fermentation broth (Amberlite® IRA120) and (3) adsorption of lactic acid by anion exchange (Amberlite® IRA67). At the end of the purification process, 80.4% (w/w) D-lactic acid was recovered with 91.8% (w/w) purity, indicating the effectiveness of the developed downstream process. Furthermore, a clear yellowish solid polymer with a molecular weight of 3010 Da was obtained, suitable for applications in biomedical and agricultural sectors.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Research Group
ID Code:84142
Publisher:Elsevier

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