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Saturated fat-induced changes in S-f 60-400 particle composition reduces uptake of LDL by HepG2 cells

Jackson, K.G., Maitin, V., Leake, D.S., Yaqoob, P. and Williams, C.M. (2006) Saturated fat-induced changes in S-f 60-400 particle composition reduces uptake of LDL by HepG2 cells. Journal of Lipid Research, 47 (2). pp. 393-403. ISSN 0022-2275

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To link to this item DOI: 10.1194/jlr.M500382-JLR200


The ability of human postprandial triacylglycerol-rich lipoproteins (TRLs), isolated after meals enriched in saturated fatty acids (SFAs), n-6 PUFAs, and MUFAs, to inhibit the uptake of I-125-labeled LDL by the LDL receptor was investigated in HepG2 cells. Addition of TRLs resulted in a dose-dependent inhibition of heparin-releasable binding, cell-associated radioactivity, and degradation products of I-125-labeled LDL (P < 0.001). SFA-rich Svedberg flotation rate (S-f) 60-400 resulted in significantly greater inhibition of cell-associated radioactivity than PUFA-rich particles (P = 0.016) and total uptake of I-125-labeled LDL compared with PUFA- and MUFA-rich particles (P = 0.02). Normalization of the apolipoprotein (apo)E but not apoC-III content of the TRLs removed the effect of meal fatty acid composition, and addition of an anti-apoE antibody reversed the inhibitory effect of TRLs on the total uptake of I-125-labeled LDL. Real time RT-PCR showed that the SFA-rich Sf 60-400 increased the expression of genes involved in hepatic lipid synthesis (P < 0.05) and decreased the expression of the LDL receptor-related protein 1 compared with MUFAs (P = 0.008). In conclusion, these findings suggest an alternative or additional mechanism whereby acute fat ingestion can influence LDL clearance via competitive apoE-dependent effects of TRL on the LDL receptor.-Jackson, K. G., V. Maitin, D. S. Leake, P. Yaqoob, and C. M. Williams. Saturated fat-induced changes in Sf 60 400 particle composition reduces uptake of LDL by HepG2 cells.

Item Type:Article
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
Life Sciences > School of Biological Sciences
Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
ID Code:13284
Uncontrolled Keywords:polyunsaturated fatty acids, monounsaturated fatty acids, apolipoprotein E, apolipoprotein C-III, low density lipoprotein, receptor, low density lipoprotein receptor-related protein 1, real-time, reverse transcription polymerase chain reaction, hepatic lipid, metabolism, Svedberg flotation rate, LOW-DENSITY LIPOPROTEINS, TRIACYLGLYCEROL-RICH LIPOPROTEINS, RECEPTOR-MEDIATED UPTAKE, APOLIPOPROTEIN-E, CULTURED-CELLS, HEP-G2, CELLS, LINE HEPG2, APO-B, BINDING, ACIDS
Publisher:American Society for Biochemistry and Molecular Biology

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