Preparation of (–)-epigallocatechin gallate from commercial green tea by caffeine precipitation and solvent partitionCopeland, E. L., Clifford, M. N. and Williams, C. M. (1998) Preparation of (–)-epigallocatechin gallate from commercial green tea by caffeine precipitation and solvent partition. Food Chemistry, 61 (1-2). pp. 81-87. ISSN 0308-8146 Full text not archived in this repository. It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing. To link to this item DOI: 10.1016/S0308-8146(97)00111-8 Abstract/SummaryA method has been developed which enables the easy and inexpensive preparation of gram quantities of (–)-epigallocatechin gallate from green tea (Camellia sinensis). A decaffeinated aqueous brew of commercial green tea is treated with caffeine (30 m ). The precipitate is redissolved after decaffeination with chloroform and further purified by solvent partition with ethyl hexanoate and propyl acetate. Commercial leaf (25 g) yields 400 mg (–)-epigallocatechin gallate at better than 80% purity, as judged by reversed phase HPLC.
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