Article

Needs, S. H., Pivetal, J., Hayward, J., Kidd, S. P., Lam, H., Diep, T., Gill, K., Woodward, M., Reis, N. M. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2023) Moving microcapillary antibiotic susceptibility testing (mcAST) towards the clinic: unravelling kinetics of detection of uropathogenic E. coli, mass-manufacturing and usability for detection of urinary tract infections in human urine. Sensors and Diagnostics. ISSN 2635-0998 doi: https://doi.org/10.1039/d2sd00138a

Diep, T. T., Needs, S. H., Bizley, S. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2022) Rapid bacterial motility monitoring using inexpensive 3D-printed openflexure microscopy allows microfluidic antibiotic susceptibility testing. Micromachines, 13 (11). 1974. ISSN 2072-666X doi: https://doi.org/10.3390/mi13111974

Long, M. M., Diep, T. T., Needs, S. H., Ross, M. J. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2022) PiRamid: A compact Raspberry Pi imaging box to automate small-scale time-lapse digital analysis, suitable for laboratory and field use. HardwareX, 12. e00377. ISSN 24680672 doi: https://doi.org/10.1016/j.ohx.2022.e00377

Needs, S. H., Saiprom, N., Rafaque, Z., Imtiaz, W., Chantratita, N., Runcharoen, C., Thammachote, J., Anun, S., Peacock, S. J., Ray, P. ORCID: https://orcid.org/0000-0001-8375-8279, Andrews, S. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2022) Miniaturised broth microdilution for simplified antibiotic susceptibility testing of Gram negative clinical isolates using microcapillary devices. Analyst (15). pp. 3385-3614. ISSN 0003-2654 doi: https://doi.org/10.1039/D2AN00305H

Needs, S. H., Bootman, M. D., Grotzke, J. E., Kramer, H. B. and Allman, S. A. (2022) Off-target inhibition of NGLY1 by the poly-caspase inhibitor Z-VAD-fmk induces cellular autophagy. The FEBS Journal, 289 (11). pp. 3115-3131. ISSN 1742-464X doi: https://doi.org/10.1111/febs.16345

Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Sirivisoot, S. ORCID: https://orcid.org/0000-0002-5809-6936, Jegouic, S., Prommool, T., Luangaram, P., Srisawat, C., Sriraksa, K., Limpitikul, W., Mairiang, D. ORCID: https://orcid.org/0000-0002-2743-0145, Malasit, P., Avirutnan, P. ORCID: https://orcid.org/0000-0003-0053-3045, Puttikhunt, C. and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X (2022) Smartphone multiplex microcapillary diagnostics using Cygnus: development and evaluation of rapid serotype-specific NS1 detection with dengue patient samples. PLOS Neglected Tropical Diseases, 16 (4). e0010266. ISSN 1935-2735 doi: https://doi.org/10.1371/journal.pntd.0010266

Donmez, S. I., Needs, S. H., Osborn, H. M. I., Reis, N. M. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2022) Label-free 1D microfluidic dipstick counting of microbial colonies and bacteriophage plaques. Lab on a Chip, 22 (15). pp. 2820-2831. ISSN 1473-0197 doi: https://doi.org/10.1039/d2lc00280a

Reis, N. M. ORCID: https://orcid.org/0000-0002-8706-6998, Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Jegouic, S. M., Gill, K. K., Sirivisoot, S., Howard, S., Kempe, J., Bola, S., Al-Hakeem, K., Jones, I. M. ORCID: https://orcid.org/0000-0002-7738-2516, Prommool, T., Luangaram, P., Avirutnan, P., Puttikhunt, C. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2021) Gravity-driven microfluidic siphons: fluidic characterization and application to quantitative immunoassays. ACS Sensors, 6 (12). pp. 4338-4348. ISSN 2379-3694 doi: https://doi.org/10.1021/acssensors.1c01524

Needs, S., Osborn, H. and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X (2021) Counting bacteria in microfluidic devices: smartphone compatible ‘dip-and-test’ viable cell quantitation using resazurin amplified detection in microliter capillary arrays. Journal of Microbiological Methods, 187. 106199. ISSN 0167-7012 doi: https://doi.org/10.1016/j.mimet.2021.106199

Needs, S., Donmez, S. I. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2021) Direct microfluidic antibiotic resistance testing in urine with smartphone capture: significant variation in sample matrix interference between individual human urine samples. RSC Advances, 11 (60). pp. 38258-38263. ISSN 2046-2069 doi: https://doi.org/10.1039/D1RA06867A

Dönmez, S. İ., Needs, S. H. ORCID: https://orcid.org/0000-0003-3407-9637, Osborn, H. M. I. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2020) Label-free smartphone quantitation of bacteria by darkfield imaging of light scattering in fluoropolymer micro capillary film allows portable detection of bacteriophage lysis. Sensors and Actuators B: Chemical, 323. p. 128645. ISSN 0925-4005 doi: https://doi.org/10.1016/j.snb.2020.128645

Needs, S., Donmez, S. I., Bull, S., McQuaid, C., Osborn, H. M. I. and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X (2020) Challenges in microfluidic and point-of-care phenotypic antimicrobial resistance tests. Frontiers in Mechanical Engineering, 6. ISSN 2297-3079 doi: https://doi.org/10.3389/fmech.2020.00073

Needs, S., Bull, S., Bravo, J., Walker, S. ORCID: https://orcid.org/0000-0001-5942-1836, Little, G., Hart, J. and Edwards, A. ORCID: https://orcid.org/0000-0003-2369-989X (2020) Remote videolink observation of model home sampling and home testing devices to simplify usability studies for point-of-care diagnostics. Wellcome Open Research, 5 (174). ISSN 2398-502X doi: https://doi.org/10.12688/wellcomeopenres.16105.1

Needs, S. H., Diep, T. T., Bull, S. P., Lindley-Decaire, A., Ray, P. and Edwards, A. D. (2019) Exploiting open source 3D printer architecture for laboratory robotics to automate high-throughput time-lapse imaging for analytical microbiology. PLOS ONE, 14 (11). e0224878. ISSN 1932-6203 doi: https://doi.org/10.1371/journal.pone.0224878

Report

Bull, S., Needs, S. and Edwards, A., (2020) Building open lab hardware to tackle antimicrobial resistance. Open Research Case Studies. Report. University of Reading doi: https://doi.org/10.17864/1926.89600