Rainey-Smith, S., Schroetke, L.W., Bahia, P., Fahmi, A., Skilton, R., Spencer, J.P.E.
ORCID: https://orcid.org/0000-0003-2931-7274, Rice-Evans, C., Rattray, M. and Williams, R.J.
(2008)
Neuroprotective effects of hesperetin in mouse primary neurones are independent of CREB activation.
Neuroscience Letters, 438 (1).
pp. 29-33.
ISSN 0304-3940
Full text not archived in this repository.
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To link to this item DOI: 10.1016/j.neulet.2008.04.056
Abstract/Summary
Dietary flavonoids, including the citrus flavanone hesperetin, may have stimulatory, effects on cytoprotective intracellular signalling pathways. In primary mouse cortical neurone cultures, but not SH-SY5Y human neuroblastoma cells or human primary dermal fibroblasts (Promocells), hesperetin (100-300 nM, 15 min) caused significant increases in the level of ERK1/2 phosphorylation, but did not increase CREB phosphorylation. Administration of hesperetin for 18 h did not alter gene expression driven by the cyclic AMP response element (CRE), assessed using a luciferase reporter system, but 300 nM hesperetin partially reversed staurosporine-induced cell death in primary neurones. Our data show that hesperetin is a neuroprotective compound at concentrations where antioxidant effects are unlikely to predominate. The effects of hesperetin are cell-type dependent and, unlike the flavanol (-)epicatechin, neuroprotection in vitro is not associated with enhanced CREB phosphorylation or CRE-mediated gene expression. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
Item Type: | Article |
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Refereed: | Yes |
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Divisions: | Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR) Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology |
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ID Code: | 13652 |
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Uncontrolled Keywords: | flavonoid, neurodegeneration, MAP kinase, luciferase reporter, GREEN TEA CATECHINS, CORTICAL-NEURONS, AGLYCONE HESPERETIN, C-JUN, FLAVONOIDS, QUERCETIN, KINASE, MEMORY, METABOLITES, NARINGENIN
Animals
Antioxidants/pharmacology/therapeutic use
Brain/*drug effects/metabolism
Cell Death/drug effects/physiology
Cells, Cultured
Cyclic AMP Response Element-Binding Protein/*drug
effects/genetics/metabolism
Dose-Response Relationship, Drug
Enzyme Activation/drug effects/physiology
Enzyme Inhibitors/pharmacology
Extracellular Signal-Regulated MAP Kinases/drug effects/metabolism
Flavonoids/pharmacology/therapeutic use
Gene Expression Regulation/drug effects/genetics
Hesperidin/*pharmacology/therapeutic use
Humans
Mice
Neurodegenerative Diseases/*drug therapy/metabolism/physiopathology
Neurons/*drug effects/metabolism
Neuroprotective Agents/*pharmacology/therapeutic use
Oxidative Stress/drug effects/physiology
Phosphorylation/drug effects
Transcriptional Activation/drug effects/genetics
Tumor Cells, Cultured |
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Publisher: | Elsevier |
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Date Deposited: | 26 Oct 2010 16:01 | Date item deposited into CentAUR |
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Last Modified: | 23 Jun 2024 05:38 | Date item last modified |
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