Accessibility navigation


Glu298Asp (rs1799983) polymorphism influences postprandial vascular reactivity and the insulin response to meals of varying fat composition in postmenopausal women: findings from the randomized, controlled DIVAS-2 study

Rathnayake, K. M., Weech, M. ORCID: https://orcid.org/0000-0003-1738-877X, Lovegrove, J. A. ORCID: https://orcid.org/0000-0001-7633-9455 and Jackson, K. G. ORCID: https://orcid.org/0000-0002-0070-3203 (2021) Glu298Asp (rs1799983) polymorphism influences postprandial vascular reactivity and the insulin response to meals of varying fat composition in postmenopausal women: findings from the randomized, controlled DIVAS-2 study. Journal of Nutrition, 151 (4). pp. 848-856. ISSN 1541-6100

[img]
Preview
Text - Accepted Version
· Please see our End User Agreement before downloading.

365kB

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1093/jn/nxaa394

Abstract/Summary

Background: Previous acute studies suggest the Glu298Asp polymorphism (rs1799983) may influence vascular reactivity in response to long-chain n-3 polyunsaturated fat (PUFA) intake. However, the effects of this genotype on postprandial vascular function following meals rich in saturated (SFA), n-6 PUFA and monounsaturated (MUFA) fats are unclear. Objective: This study determined the impact of the Glu298Asp polymorphism on changes in vascular function and cardiometabolic risk biomarkers in response to sequential meals of varying fat composition. Methods: In a randomized, double-blind, cross-over, acute study, 32 postmenopausal women (mean±SD age 58±5 y; BMI 25.9±4.1 kg/m2) consumed mixed meals (breakfast: 0 min, 50 g fat; lunch: 330 min, 30 g fat) containing SFA, n-6 PUFA or MUFA on 3 occasions. Blood samples for cardiometabolic disease risk markers and real-time measures of vascular reactivity (including flow-mediated dilatation (FMD, primary outcome)) were collected/performed before and regularly for 480 min after breakfast. Participants were retrospectively genotyped for the Glu298Asp (rs1799983) polymorphism. Data were analysed using linear mixed models. Results: For the postprandial %FMD response, a test fat x genotype interaction was observed for the area under the curve (AUC; P=0.019) but not incremental AUC, with the AUC being ~24% greater after MUFA than SFA and n-6 PUFA-rich meals in the Glu298 homozygotes (P≤0.026). Test fat x genotype interactions were also evident for postprandial insulin (P≤0.005), with the MUFA-rich meals demonstrating significantly higher AUC (12.8%/14.9%), incremental AUC (14.6%/20.0%) and maximum concentration (20.0%/34.5%) versus the SFA and n-6 PUFA-rich meals (respectively) in Asp298 carriers (P<0.05). Genotype did not influence other study outcome measures in response to the test fats. Conclusion: Our findings suggest the Glu298Asp polymorphism may represent a potential determinant of the inter-individual variability in postprandial responsiveness of %FMD and insulin to acute meal fat composition in postmenopausal women. Further studies are required to confirm these observations.

Item Type:Article
Refereed:Yes
Divisions:Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
Interdisciplinary Research Centres (IDRCs) > Institute for Food, Nutrition and Health (IFNH)
Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group
ID Code:93965
Publisher:American Society for Nutrition

Downloads

Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation