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Involvement of ERK, Akt and JNK signalling in H2O2-induced cell injury and protection by hydroxytyrosol and its metabolite homovanillic alcohol

Incani, A., Deiana, M., Corona, G., Vafeiadou, K., Vauzour, D., Dessi, M. A. and Spencer, J. (2010) Involvement of ERK, Akt and JNK signalling in H2O2-induced cell injury and protection by hydroxytyrosol and its metabolite homovanillic alcohol. Molecular Nutrition & Food Research, 54 (6). pp. 788-796. ISSN 1613-4125

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To link to this article DOI: 10.1002/mnfr.200900098

Abstract/Summary

The olive oil polyphenol, hydroxytyrosol (HT), is believed to be capable of exerting protection against oxidative kidney injury. In this study we have investigated the ability of HT and its O-methylated metabolite, homovanillic alcohol (HVA) to protect renal cells against oxidative damage induced by hydrogen peroxide. We show that both compounds were capable of inhibiting hydrogen peroxide-induced kidney cell injury via an ability to interact with both MAP kinase and PI3 kinase signalling pathways, albeit at different concentrations. HT strongly inhibited death and prevented peroxide-induced increases in ERK1/2 and JNK1/2/3 phosphorylation at 0.3 microM, whilst HVA was effective at 10 microM. At similar concentrations, both compounds also prevented peroxide-induced reductions in Akt phosphorylation. We suggest that one potential protective effect exerted by olive oil polyphenols against oxidative kidney cell injury may be attributed to the interactions of HT and HVA with these important intracellular signalling pathways.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group
Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
ID Code:18548
Uncontrolled Keywords:Animals Cell Survival/drug effects Cells, Cultured *Cytoprotection Extracellular Signal-Regulated MAP Kinases/*physiology Homovanillic Acid/*pharmacology Hydrogen Peroxide/*toxicity JNK Mitogen-Activated Protein Kinases/*physiology Phenylethyl Alcohol/*analogs & derivatives/pharmacology Proto-Oncogene Proteins c-akt/*physiology Signal Transduction/*drug effects Swine
Publisher:Wiley

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