Exploring the acute and chronic effects of a multi-strain probiotic supplement on cognitive function and mood in healthy older adults: A randomised controlled trial

Eastwood, J., van Hemert, S., Stolaki, M., Williams, C. ORCID: https://orcid.org/0000-0003-4452-671X, Walton, G. ORCID: https://orcid.org/0000-0001-5426-5635 and Lamport, D. ORCID: https://orcid.org/0000-0002-4592-0439 (2025) Exploring the acute and chronic effects of a multi-strain probiotic supplement on cognitive function and mood in healthy older adults: A randomised controlled trial. American Journal of Clinical Nutrition, 121 (6). pp. 1268-1280. ISSN 1938-3207 doi: 10.1016/j.ajcnut.2025.04.002

Abstract/Summary

Background: Ageing, is associated with a decline in cognitive function and vulnerability to depression. Probiotic supplements have shown beneficial effects on cognition and mood in clinical populations, but the potential benefit for healthy older adults experiencing age-related decline in cognition remains unclear. Objectives: The primary aim of the present work was to explore the effect of a chronic (long-term) multi-species probiotic intervention on cognition in healthy ageing adults. Secondary aims included exploring the chronic effect on mood outcomes and gut microbiota community, as well as a novel investigation into the acute effect of supplementation on cognition and mood. Method: The study employed a randomised, placebo-controlled, cross-over trial in 30 healthy older adults to explore the acute (1 day) and chronic (8 weeks) effects of a probiotic supplement (Ecologic® Barrier) on cognitive domains of memory and executive function, alongside mood measures of stress, anxiety, depression, and cognitive reactivity to sad mood. 16s rRNA sequencing of stool samples was also performed pre- and post- chronic intervention to assess potential effects on the gut microbiota. Results: Acute probiotic supplementation was associated with faster reaction times on cognitively demanding trials during a task of executive function [-64.91ms, CI -115.70, -14.15]. Chronic supplementation was associated with improvement in cognitive biases such as hopelessness [-0.97, CI -1.72, -0.23], rumination [-1.58, CI -2.86, -0.29] and aggression [-1.57, CI -2.63, -0.51] that contribute to reactivity to sad mood and therefore vulnerability to depression, and may improve executive function under higher cognitive demand [0.43%, CI -0.53%, 1.38%]. Conclusions: The current work provides novel evidence for an acute effect of probiotics on reaction times during executive function, which should be replicated in future work. Additionally, this work replicates previous findings of improved cognitive reactivity to sad mood following chronic probiotic supplementation, indicating probiotics may reduce the risk of developing depression in a healthy ageing population. This trial was registered at https://clinicaltrials.gov/study/NCT04951687.

Item Type	Article
URI	https://centaur.reading.ac.uk/id/eprint/122668
Identification Number/DOI	10.1016/j.ajcnut.2025.04.002
Refereed	Yes
Divisions	Life Sciences > School of Psychology and Clinical Language Sciences > Department of Psychology Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group Life Sciences > School of Psychology and Clinical Language Sciences > Nutrition and Health
Publisher	American Society for Nutrition
Download/View statistics	View download statistics for this item